Local and international companies are being lured by the area's untouched gold riches, but groups warn of potential impacts to watersheds and communities.
Unionized miners will hold a day-long strike on Tuesday in a show of solidarity with ongoing protests over the high cost of living and extreme inequality in the country.
Glencore's funding gets it an 11.58% stake in Group Eleven Resources, which co-owns the Stonepark deposit with Irish gold and zinc junior Arkle Resources.
The upgrade is expected to increase average annual gold production at the Ghana mine by between 75,000 and 100,000 ounces for the first five years, starting in 2020.